New Journal of Chemistry (2020).
Lipophilicity plays a significant role as a determinant of biological properties. The reversed-phase high-performance thin layer chromatography (HP-TLC) was applied to determine the logP values of chelidonine, allocryptopine, sanguinarine chloride and chelerythrine chloride at three different pH values: 3.0, 7.4 and 10.0. The mobile phase was a mixture of acetonitrile and water with different concentrations of acetonitrile (50-75% vol.). The retention factors, Rm0 values, for the compounds with known logP and for the analyzed substances were determined. The logP values obtained experimentally were compared with the theoretical data obtained by using known algorithms (miLogP, xLogP, AlogPs). Additionally, logD values of examined substances were determined. The highest logP value was observed for chelerythrine chloride in the alkaline environment, while the lowest one was noted for chelidonine at acidic pH.
