Since the outbreak of COVID-19, the industry has reached a peak as terms such as “national nucleic acid test” has become increasingly popular and molecular diagnosis has been widely known by the general public. Most molecular diagnostic experiments target nucleic acid (DNA or RNA) of the sample as the test object, and the efficient acquisition of high-quality nucleic acid is the basis and key to the success of most molecular diagnostic experiments.
For different application scenarios, the pain points and solutions of nucleic acid extraction are quite different from each other. Below is a review of four main nucleic acid extraction application scenarios and the corresponding solutions.
Scenario 1: Nucleic acid extraction of pathogens
After the outbreak, with the growing testing demand and overload work, the supply of large commercial kits and raw materials at low cost as well as a high-throughput automatic nucleic acid extraction platform has great appeal to users working on nucleic acid testing.
With the rapid development of automated instruments, nucleic acid extraction solutions based on magnetic bead method are already commonly used in molecular diagnosis, especially nucleic acid detection, and greatly assist in realizing high-throughput automated processes.
A magnetic bead kit called sbeadex shows the advantages that, through the innovative two-step binding method, the drying step is omitted, and the wash solution is water-based, which effectively reduces the influence of organic solvents in the final purified nucleic acid sample on downstream experiments. The principle of sbeadex kit is that it uses a surface-modified superparamagnetic magnetic particle and is double-coated. The innovative two-step binding mechanism of magnetic beads enables sbeadex kits to replace all the existing commercial purification kits that are based on the magnetic bead method.
Scenario 2: Several different kits are required for distinct types of samples
Blood samples of EDTA, heparin, citrate anticoagulant, and buffy coat and various cell tissues are often used in molecular diagnostic experiments. Different samples usually need different extraction procedures and even different kits to complete. Sometimes, even if the same kit is used, the amount of different components used is not the same. It is often an embarrassing situation that the whole kit is discarded because some components have been used up, resulting in a large amount of reagent waste.
To solve the above issues, the sbeadex blood nucleic acid extraction kit provides a universal extraction procedure. The reagent is ready to use, easy to operate, and easy to extract high-quality DNA and RNA from the above blood and tissue cells, which can be used for all downstream applications related to molecular biology, including PCR, qPCR, KASP, BHQplus probe PCR, first and second generation sequencing of cloning enzyme digestion, and gene chip, etc.
Scenario 3: Rapid nucleic acid extraction
Most PCR-based downstream applications do not require high nucleic acid quality but require a fast and easy nucleic acid extraction process in order to increase the flux and speed up the detection process. This is where a quick extraction kit (which can be done in about five minutes) is very attractive. Some quick extraction kits only take 5-8 minutes to complete the nucleic acid extraction process, maximizing the efficiency and simplifying the process. When combined with the automated workstation, it can easily accomplish nucleic acid extraction, which used to be a rate-limiting step.
Scenario 4: Extraction of high molecular weight and long strand DNA for third-generation sequencing and bacterial artificial chromosome (BAC) library construction
Real-time (SMRT) sequencing established in 2004 is the earliest widely used long-read sequencing technology (third-generation sequencing). SMRT sequencing can produce Reads up to about 200kb to identify genetic variants and further study their gene functions. In recent years, the accuracy of sequencing has been significantly improved, bringing great benefits to scientific research.
At this point, it’s essential to consider whether the nucleic acid quality can meet the requirements of such long reads. In fact, the technologies applied are not only single-molecule long-read sequencing, bacterial artificial chromosome (BAC) library construction, recombinant chromosome, plasmid, southern blot, etc. In order to solve the problems of highly repetitive regions of the genome, structural variation of large fragments, and conformation of large fragments, it is also particularly important to prepare DNAs with high quality and high molecular weight.
The same kit can be used for the lysis and digestion of all types of samples. After centrifugation, proteins and other precipitates are removed, while the supernatant is retained and other impurities are washed away. The total nucleic acid is precipitated by centrifugation, and the target nucleic acid is obtained by removing RNA or DNA with RNase or DNase as required.
Related Products:
| Name | CAS | Molecular Weight | Description |
| Superparamagnetic iron oxide microbeads | – | – | Products include various-sized magnetic beads with different coupling groups for the conjugation of biomolecules. |
| COVID-19 | – | – | Products include disinfectants, SARS-CoV-2 inhibitors, testing reagents, and vaccine-related products needed to treat COVID-19. |
| Heparin | 9005-49-6 | 1134.93 | Heparin, a highly sulfated glycosaminoglycan, is widely used as an injectable anticoagulant, and has the highest negative charge density of any known biological molecule. |
| Citrate | 2572-63-6 | 189.10 | The principle of citrate anticoagulation is to combine calcium ions in the blood to form chelates, so that calcium ions lose their coagulation function. |